Detecting HIV-1 integration by repetitive-sampling Alu-gag PCR
نویسندگان
چکیده
منابع مشابه
Quantifying integrated SIV-DNA by repetitive-sampling Alu-gag PCR
OBJECTIVES Although antiretroviral therapy (ART) effectively suppresses HIV-1 replication, it does not eradicate the virus and ART interruption consistently results in rebound of viraemia, demonstrating the persistence of a long-lived viral reservoir. Several approaches aimed at reducing virus persistence are being developed, and accurate measurements of the latent reservoir (LR) are necessary ...
متن کاملQuantification of integrated HIV DNA by repetitive-sampling Alu-HIV PCR on the basis of poisson statistics.
BACKGROUND Quantification of integrated proviral HIV DNA by repetitive-sampling Alu-HIV PCR is a candidate virological tool to monitor the HIV reservoir in patients. However, the experimental procedures and data analysis of the assay are complex and hinder its widespread use. Here, we provide an improved and simplified data analysis method by adopting binomial and Poisson statistics. METHODS ...
متن کاملHIV-1 GAG Sequence Summaries
HIVU53175 HIVCA9 U53175 422 bp gag Braaten, D. JVI 70, 4220 (1996) Comment: This study compared the five lineages of primate immunodeficiency viruses and found that only HIV-1 group M requires cyclophilin A for replication. HIV-1 gag binds to cyclophilin A and incorporates it into virions. If this process is disrupted, virion infectivity is inhibited. Cloned isolates from clades A, B, and D of ...
متن کاملALU Augmentation for MPEG-4 Repetitive Padding
In this paper we augment a general purpose ALU with an extra functionality a repetitive padding operation. The proposed solution enables the processor to perform the time exhaustive MPEG-4 padding algorithm in real time. At trivial hardware costs of a few hundred 2x2 AND-OR (or equivalent) logical gates, we achieve an order of magnitude speed-up when compared to software running on a general pu...
متن کاملIntegration site preferences of the Alu family and similar repetitive DNA sequences.
Numerous flanking nucleotide sequences from two primate interspersed repetitive DNA families have been aligned to determine the integration site preferences of each repetitive family. This analysis indicates that both the human Alu and galago Monomer families were preferentially inserted into short d(A+T)-rich regions. Moreover, both primate repeat families demonstrated an orientation specific ...
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ژورنال
عنوان ژورنال: Methods
سال: 2009
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2009.01.002